Nuestro grupo organiza más de 3000 Series de conferencias Eventos cada año en EE. UU., Europa y América. Asia con el apoyo de 1.000 sociedades científicas más y publica más de 700 Acceso abierto Revistas que contienen más de 50.000 personalidades eminentes, científicos de renombre como miembros del consejo editorial.
Revistas de acceso abierto que ganan más lectores y citas
700 revistas y 15 000 000 de lectores Cada revista obtiene más de 25 000 lectores
S. V. Taralkar and S. Chattopadhyay
Ursolic and betulinic acids are very useful nutraceuticals available in herbs. Their quantitative estimation from the solvent extracts of the herbs is a great challenge. So far all the chromatographic methods available for identification and quantification of these acids are distinctly different, meaning thereby, each acid would require a separate method. Reverse phase (RP-HPLC) method is developed for determination of ursolic acid and betulinic acid from their methanol extract of Vitex negundo Linn leaves. Analysis was carried out using Waters’ symmetry C-18 column with acetonitrile: methanol (80:20) as isocratic elution mode with UV detection (λ=210 nm). The method is pretty linear for ursolic acid in the range of 0.01-0.1 mg/ml (R2 = 0.9961) and for betulinic acid in the range of 0.003-0.018 mg/ml (R2 = 0.999). The peaks of ursolic acid and betulinic acid were confirmed by LC-MS. The method was validated by mixing these acids standards in methanol and found that it is accurate, sensitive and has a good reproducibility.