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Zeng WW, Yanovski AJ
Melanocortin 3 receptor (MC3R) is involved in the regulation of energy homeostasis. Study on mouse MC3R promoter has been performed following the determination of its transcription starting site, mouse MC3r deletion constructs were built by sub cloning into pGL3 vector, then the gene reporter assay of the seven deletion constructs was tested by Dual Luciferase Assay.
On the other hand, phosphorylation and glycosylation play an important role in the transcription regulation. The global distribution of phosphorylation and glycosylation on transcription factor could be found in open source database.
In this report, relative luciferase activity and the distribution of phosphorylation-glycosylation sites on promoter model transcription factor were plotted, for the first time, on the same chart, within a 3.2 kb upstream the methinoin ATG, the two trend lines of the relative luciferase activity and the distribution of phophorylation-glycosylation sites, they were found tend to be negative reciprocal.