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Dr. Pawolski Vador
Mass spectrometry (MS) techniques have revolutionized metabolomics research, enabling comprehensive analysis of small molecules present in biological systems. This abstract highlights the advancements in mass spectrometry techniques for metabolomics research, their applications, and their impact on understanding cellular metabolism and disease mechanisms. High-resolution mass spectrometry (HRMS) offers improved resolution and mass accuracy, enabling the identification and quantification of a wide range of metabolites. Ion mobility spectrometry (IMS) coupled with MS enhances peak capacity and facilitates the characterization of isomeric metabolites. Tandem mass spectrometry (MS/MS) techniques provide structural elucidation of metabolites, while imaging mass spectrometry (IMS) allows the spatial mapping of metabolites in tissues and cells. Multidimensional chromatography techniques combined with MS enhance metabolite separation and coverage. These advancements have led to a deeper understanding of metabolomics profiles, metabolic pathways, and disease biomarkers. Continued developments in mass spectrometry instrumentation and data analysis algorithms hold immense promise for further advancements in metabolomics research and its application in personalized medicine.