Revista de terapia y enfermedades infecciosas

Abstracto

Antiviral Activity of the Hemolymph of Podalia sp and M. Albicolis (Lepidoptera: Megalopigydae)

Carvalho ND, Curti SP, Oliveira MI, Rofatto HK, Figueiredo CA, Villar KS, Magnelli RF, Mendonca RZ

Potent antiviral activity against measles, influenza, picornavirus and herpès simplex viruses was observed in the hemolymph of Podalia sp and M. Albicolis (Lepidoptera: Megalopigydae). The antiviral proteins responsible for this activity were isolated by gel filtration chromatography using à gel filtration column system (Superdex 75) and further fractionated using a Resource-Q ion exchange column system. Experiments with the semi-purified protein led to a 128-fold reduction in picornavirus production, 64-fold reduction in measles virus production and a 32-fold reduction in influenza virus replication. qPCR showed a significantly lower level of herpes virus transcription. In addition no citotoxicity and genotoxicity effect was observed for Vero cells, suggesting a very interesting potential antiviral activity.