Revista de patología clínica y experimental

Abstracto

Histopathological Study of Acute Toxicity of Adonis Aestivalis (Summer Pheasant's Eye) in Rabbits

Rahim Hobbenaghi, Javad Javanbakht, Moharam Kamrani, Ali Bashiri Dezfouli, Mehdi Aghamohammad Hassan and Mohamad Zamani-ahmadmahmudi

Abstract
The aim of this study was to evaluate the acute toxicity of the hydroalcolic extract obtained from flowers and
leaves of A. aestivalis in New Zealand White rabbits. In this study Sixteen New Zealand White rabbits (weighing 1700-
2200 g) were tested for 14 days. The animals were randomly divided into 2 groups (treatment and control) which every
group contained 8 rabbits. A. aestivalis (summer pheasant’s eye) was collected over multiple years, under different
growing conditions, and at various stages of maturity, dried, and administered to rabbits to evaluate the toxicity of A.
aestivalis. On microscopic examination, Severe hemorrhage, myocardial degeneration and necrosis in cardiac cells,
severe congestion and swelling of hepatocytes in liver, spread interstitial alveolar edema, congestion of pulmonary
arteries, medial hyperplasia and emphysema in lungs, PVC (pre-vascular cuffing), chromatolysis, neurophagy and
focal gliosis in brain, severe swelling of tubular endothelial cells, congestion of glomeruli, hyaline casts and severe
tubular necrosis in kidney, papillary hyperplasia of mucus layer, and mucous secretions into the intestine lumen were
observed. No pathological were found in other organs as well. Although Adonis spp. contain cardiac glycosides,
cardiac lesions have not previously been described in laboratory animals associated with consumption of adonis, and
this is the first report of adonis toxicosis in Iran. Effectively, these results demonstrate the presence of acute toxicity
due to ip treatment with A. aestivalis for 14 days in New Zealand White rabbits. However, other toxicological studies
are necessary to evaluate the total.