Epidemiología: acceso abierto

Abstracto

Molecular characterization of circulating rabies virus and knowledge attitude practice in Uganda

Michael Omodo

Introduction: Rabies is a viral disease that affects mammals and remains a public health threat in Uganda. The aim of study was to explore knowledge, Attitude, Practice of rabies and molecular epidemiology of rabies virus in Uganda.

Methodology: Knowledge, Attitude and Practices (KAP) survey about rabies, data was collected purposively based on animal biting history from veterinary departments, Medical units and selected households. Eighty-four (84) households in Ntoroko and Moyo districts. Fisher’s exact test or the Chi-square X² tests were used to analyse data. Thirty-five (35) brain tissues from dogs, cattle, goats, foxes and jackals, in Jinja, Kabale, Kabarole, Kabongo, Jinja, Moyo, Ntoroko and Namayingo were tested. Fluorescent antibody test was used to detect rabies virus, RNA was extracted using Qiagen kit, and one-step RT-PCR was performed PCR products were sequenced by automated Sanger sequencing method