Toxicología: acceso abierto

Abstracto

Resveratrol's Renal Defense: Activating HSP70 Expression for Kidney Protection in Uremic Rats

Memuna Kintzios

The serum creatinine and urea nitrogen levels were detected by Automatic Biochemical Analyzer. The pathological changes of renal tissues and the renal interstitial fibrosis were analyzed by hematoxylin-eosin and Masson, respectively. The expression of HSP70 protein in renal tissues was detected by immunohistochemistry. The expression of HSP70 and NF-κB pathway-related proteins were detected by Western blot. To further validate the protective role of resveratrol through activating HSP70 in uremic rats, HSP70 activator and HSP70 inhibitor group were used. Results. In the model group, the levels of Cr and BUN in serum were significantly increased, and the renal interstitial collagen deposition was also obviously increased. Compared with the model group, the levels of Cr and BUN in different doses of resveratrol groups were remarkably declined, and the renal interstitial collagen deposition was declined . Resveratrol also significantly improved the renal tissue lesions when compared with the model group. In renal tissues, different doses of resveratrol treatment remarkably raised HSP70 and p-IκBα expression and also remarkably declined the level of p-P65 protein. Meanwhile, the effect of 17-AAG was similar to 20 mg/kg resveratrol on NF-κB pathway-related proteins expression. After the added MKT-077 in the resveratrol treatment group, the levels of HSP70 and p-IκBα in the renal tissue were remarkably declined; however, the levels of p-P65 protein was remarkably raised. Conclusion. Resveratrol played a protective role on the kidney of uremic rats through activating HSP70 expression.