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Jing Zhang, Mao Liu, Jiaqi Zhang, Qiulai He, Kai Yang, Hongyu Wang and Wei Li
This study focused on the effect of the variation of intermediate volatile fatty acids (VFAs) caused by the change of organic load on methane production and methanogenic community shift during anaerobic digestion (AD). Eight groups with different F/I (VSfood wastes:VSsludge) were conducted, while the inoculation sludge had a same amount. Operational performance for every groups was monitored by assessing biological activity, methane production, concentration of VFAs. The major intermediate VFAs of anaerobic digestion were acetate, propionate, iso-butyrate, n-butyrate, n-valerate and iso-valerate; and acetate and n-butyrate were the most abundant components. The increasing of F/I changed the predominant VFAs type from acetic acid to n-butyric acid, while the total VFAs concentration increased. Besides, the methane production increased and then decreased, and reached the maximum (170.3 ml/g added VS-1) at F/I of 1. The methanogenic diversity was screened using the high-throughput sequencing, which shown VFAs had a significant impact on the archaeal community as the dominant archaea shifted from Methano bacterium to Methano sarcina.